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Centrifugation

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1. What is the purpose of centrifugation?

a) To separate particles of different densities

b) To purify a solution

c) To measure the pH of a solution

d) To mix two solutions together

2. What type of centrifuge is used for separating large particles, such as cells or bacteria?

a) Microcentrifuge

b) Ultracentrifuge

c) Centrifuge tube

d) Centrifuge rotor

3. What type of centrifuge is used for separating small particles, such as proteins or DNA?

a) Microcentrifuge

b) Ultracentrifuge

c) Centrifuge tube

d) Centrifuge rotor

4. What is the difference between centrifugation and sedimentation?

a) Centrifugation uses a spinning motion to separate particles, while sedimentation uses gravity to separate particles

b) Centrifugation uses a spinning motion to mix particles, while sedimentation uses gravity to mix particles

c) Centrifugation uses a spinning motion to measure pH, while sedimentation uses gravity to measure pH

d) Centrifugation uses a spinning motion to purify a solution, while sedimentation uses gravity to purify a solution

5. What is the speed of a microcentrifuge typically set at?

a) 15,000 RPM

b) 25,000 RPM

c) 50,000 RPM

d) 100,000 RPM

6. What is the speed of an ultracentrifuge typically set at?

a) 15,000 RPM

b) 25,000 RPM

c) 50,000 RPM

d) 100,000 RPM

7. How do you calculate the relative centrifugal force (RCF) of a centrifugation?

a) RCF = (radius of rotor) x (speed of rotor)

b) RCF = (radius of rotor) x (speed of rotor) x (density of sample)

c) RCF = (radius of rotor) x (speed of rotor) x (g)

d) RCF = (radius of rotor) x (speed of rotor) x (mass of sample)

8. What is the difference between a fixed angle rotor and a swing-out rotor?

a) A fixed angle rotor is used for large particles, while a swing-out rotor is used for small particles

b) A fixed angle rotor is used for small particles, while a swing-out rotor is used for large particles

c) A fixed angle rotor is used for samples that need to be centrifuged for a long time, while a swing-out rotor is used for samples that need to be centrifuged quickly

d) A fixed angle rotor is used for samples that need to be centrifuged quickly, while a swing-out rotor is used for samples that need to be centrifuged for a long time

9. What is the difference between a low speed centrifuge and a high speed centrifuge?

a) A low speed centrifuge is used for large particles, while a high speed centrifuge is used for small particles

b) A low speed centrifuge is used for small particles, while a high speed centrifuge is used for large particles

c) A low speed centrifuge is used for samples that need to be centrifuged for a long time, while a high speed centrifuge is used for samples that need to be centrifuged quickly

d) A low speed centrifuge is used for samples that need to be centrifuged quickly, while a high speed centrifuge is used for samples that need to be centrifuged for a long time

10. What is the difference between a benchtop centrifuge and a floor-standing centrifuge?

a) A benchtop centrifuge is smaller and designed to be placed on a lab bench, while a floor-standing centrifuge is larger and designed to be placed on the floor

b) A benchtop centrifuge is used for large particles, while a floor-standing centrifuge is used for small particles

c) A benchtop centrifuge is used for samples that need to be centrifuged for a short time, while a floor-standing centrifuge is used for samples that need to be centrifuged for a long time

d) A benchtop centrifuge is used for samples that need to be centrifuged quickly, while a floor-standing centrifuge is used for samples that need to be centrifuged at a lower speed

Answers:

1)a

2)b

3)a

4)a

5)b

6)d

7)c

8)d

9)c

10)a

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